BS0603 - Analytical Methods and Applied Genetics

SYNOPSIS OF MODULE

This module will provide students with in-depth knowledge of a range of biomedical techniques used in the investigation and diagnosis of disease. Students will be introduced to biosensors, spectroscopy (e.g. nuclear magnetic resonance, mass spectroscopy, flow cytometry), separation techniques (e.g. capillary electrophoresis, 2 dimensional electrophoresis, advanced chromatographic seperations) and advanced molecular genetics techniques, together with their biomedical applications. The importance of the Human Genome Project will be discussed and students will be encouraged to consider its ethical, legal and social implications. The use of bioinformatics in Biomedical Sciences will also be investigated both from a research and diagnostic point of view.
The teaching of this module will be by a combination of lectures and practical classes, with assessment taking the form of an online examination for each aspect of the module i.e. one for Analytical Methods and one for Applied Genetics.
On completion of this module students will be able to review the principles of analytical and molecular genetics techniques used in bioscience and additionally be able to critically evaluate and appraise their application and contribution to the diagnosis, understanding and treatment of human diseases.

INDICATIVE READING LIST OR OTHER LEARNING RESOURCES

Latest Editions of:
Reed, R.H., Holmes, D., Weyers, J. & Jones, A. Practical Skills in Biomolecular Sciences. Pearson.
Wilson, K. & Walker, J.M. Principles and Techniques of Practical Biochemistry. Cambridge University Press.
Johnstone, R.A.W. & Rose, M.E. Mass Spectrometry for Chemists and Biochemists. Cambridge University Press.
Strachan, T. & Reed, A.P. Human Molecular Genetics. BIOS Scientific Publishers Ltd.
Sudbery, P. Human Molecular Genetics. Longman.
Bishop, J. Transgenic Mammals. Longman.
Conor, M. & Ferguson-Smith, M. Essential Medical Genetics. Blackwell Science.
Brown, T.A. Gene Cloning and DNA Analysis. Blackwell Publishing.
Attwood T.K. & Parry-Smith, D.J. Introduction to Bioinformatics. Addison Wesley Longman.

OUTLINE SYLLABUS

Analytical Methods:
Advanced separation techniques for biomolecules: chromatographic techniques, purification of enzymes, strategies for protein purification, Flow cytometry.
Electrophoretic techniques: 2-dimensional electrophoresis, capillary electrophoresis (theory & application), electrophoresis in clinical diagnosis.
Enzymatic analysis: end-point & kinetic assays, spectrophotometry, spectrofluorimetry, HPLC techniques, coupled assay systems.
Spectroscopic methods: nuclear magnetic resonance in medicine, mass spectrometry as a mode of detection/identification, applications of fluorescence spectroscopy in biomedical sciences.
Automated analysis: autoanalysers, amino acid analysers, automated DNA sequencers.
Biosenors: enzymatic, microbial, ion-selective and amperometric biosensors.
Applied Genetics:
Genetic engineering: recombinant proteins, expression vectors, primer design, mutagenesis.
Transgenics: transgenic plants/animals and their use in biomedical research.
DNA profiling: mini/micro satellites, DNA fingerprinting, DNA profiling.
Genomics: human and other genome projects (including ethical, legal and social implications), advances in DNA sequencing.
Current/future developments: bioinformatics, mutation detection, new technologies in genetics.

AIMS OF MODULE

1. To foster a sound understanding in students of advanced analytical techniques used in biomedical sciences and other related fields.
2. To demonstrate to students how such techniques are applied from a research and diagnostic point of view in biomedical sciences and other related fields.
3. To provide students with an opportunity to advance their knowledge of molecular genetics, allowing them to understand its growing importance in biomedical sciences.
4. To introduce students to advanced molecular genetics techniques and their application in biomedical sciences.
5. To highlight to students the importance of genome projects in biological sciences and encourage them to consider the ethical, legal and social implications of the information obtained from these.
6. To enable students to consolidate their practical, analytical and interpretation skills of biochemical and genetic data

LEARNING OUTCOMES

Students will be able to:
1. Comprehend concepts and understand the fundamental principles of a range of advanced analytical techniques as applicable to biosciences. (assessed by semester 1 examination)
2. Critically review the principles of operation of a range of advanced analytical techniques and evaluate their application in bioscience. (assessed by semester 1 examination)
3. Evaluate a range of advanced molecular genetics technique and assess critically their application in biosciences. (assessed by semester 2 examination)
4. Appraise the principles of genome projects, in particular the human genome project, and evaluate the ethical, legal and social implications of such knowledge. (assessed by semester 2 examination)
5. Critically assess the contribution of genetics to the diagnosis, understanding and treatment of human disease. (assessed by semester 2 examination)
6. Carryout laboratory procedures with due regard for risk assessment/health & safety to obtain, record, collate, interpret, analyse and evaluate data. (assessed formatively and by observation in practical classes)
7. Within in the range of analytical and molecular genetics techniques studied in this module, satisfy the HPC Standards of Proficiency in relation to the practical skills required by a practicing biomedical scientist. (assessed formatively)

PREREQUISITES

BS0500 – Biotechniques & Practical Molecular Genetics or equivalent or appropriate pre-module reading.

COREQUISITE(S)

None

DISTANCE LEARNING DELIVERY

N/A

LEARNING AND TEACHING STRATEGY

Learning, which will take the form of a combination of lectures supported by practical sessions, will focus on the underlying principles of various advanced analytical and molecular genetics techniques and their application in disease investigation and diagnosis, thus building on the knowledge and skills acquired by the students in Levels 4 and 5. In addition to this structured learning, students will be expected to undertake independent self-directed learning in order to expand their knowledge above that provided in lectures and refresh their background knowledge. Analytical and interpretation skills will be fostered in the students by providing a series of short answer questions at the end of each practical, the answers for which will be provided together with formative study material on the module’s eLP and aimed at facilitating revision and providing self-evaluation.

ASSESSMENT AND FEEDBACK STRATEGY

a Summative assessment and rationale for tasks
The students’ understanding of the principles, relevance and application of the various analytical and genetics techniques will be assessed in the form of two end of semester assessments delivered via the eLP in the case of semester 1 and as an unseen examination for semester 2. Included in these assessments will be a combination of MCQs, short answer questions, calculations and essay-style questions designed to test the students’ ability to analyse and interpret experimental data similar to that was obtained in the practicals, thus enabling possible disease identification/diagnosis. The use of this style of assessment in this module will help alleviate some of the pressures associated with assessing large student numbers (>80) but will also assess students via a range of question styles with the aim of suit various different learning styles.

b. Additional formative assessment – detail of process and rationale
On completing the practical sessions, students will be provided with a related series of short questions that will assist them in ascertaining their understanding of the practical and ability to interpret the appropriate data. In addition, a range of MCQs will be posted on the module?s eLP to allow students to become familiar with the type and style of questions that will be used in the summative assessments.

c. Indication of how students will get feedback and how this will support their learning
By using formative MCQs, that can be attempted unlimited number times, scores and feedback will be available immediately thus allowing students to identify problem areas that require additional study quickly. By using online summative assessments, students will receive their grades and feedback instantaneously thus meeting the university?s prescribed turnaround time and in the case of the first assessment will assist students in assessing their knowledge and work levels.

IMPLICATIONS FOR CHOICE

None

Course info

Credits 20

Level of Study Undergraduate

Mode of Study 36 months

Location Singapore

City Singapore

Start November or April

Fee Information

Module Information

All information on this course page is accurate at the time of viewing.

Our Campus based courses starting in 2022 and 2023 will be delivered on-campus with supporting online learning content. We continue to monitor government and local authority guidance in relation to Covid-19 and we are ready and able to adjust the delivery of our education accordingly to ensure the health and safety of our students and staff.

On-campus contact time is subject to increase or decrease in line with any additional restrictions, which may be imposed by the Government or the University in the interest of maintaining the health and safety and wellbeing of students, staff, and visitors. This could potentially mean increased or fully online delivery, should such restrictions on in-person contact time be required.

 

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